Hepatoprotective Potentials of the Extract and Fractions of Brenania brieyi (De Wild) (Rubiaceae) against Carbon tetrachloride Induced Liver Damage in Rats
DOI:
https://doi.org/10.59493/advpbl/2024.1.3Keywords:
Brenania brieyi; Silymarin; Hepatoprotective potentials; Carbon tetrachloride; liver damage; liver enzymes; biochemical parametersAbstract
Brenania brieyi (De Wild), a plant from the Rubiaceae family, has been traditionally used in treating various illnesses, especially for treating liver disorders. This study aims to verify the hepatoprotective properties of the extract and fractions of B. brieyi against carbon tetrachloride-induced liver damage in rats and compare it with the positive control (Silymarin) using the protective model. The plant material was extracted using cold maceration and fractionated with n-hexane, ethyl acetate and methanol to get different fractions. Phytochemical constituents were determined using the Gas Chromatography-Flame Ionization Detection (GC-FID) method. Hepatoprotective activity and Histopathology studies were evaluated using standard procedures in determining biochemical parameters such as alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT), Acid Phosphatase (ACP), albumin bilirubin, urea, creatinine, and total protein. The extract and fractions were shown to be rich in flavonoids, glycosides, alkaloids, saponins, tannins and steroids. The results demonstrated that 200 mg/kg of the extract significantly (p<0.05) reduced the levels of ALT, AST, and ALP from (46.60±2.08, 74.80±1.59 and 97.40±1.32 IU/L) to (23.20±1.02, 38.40±0.51 and 46.40±0.81 IU/L) respectively compared to (24.20±0.66, 37.20±1.15 and 48.40±0.81 IU/L) reduction obtained from the positive control (Silymarin 25 mg/Kg), indicating its hepatoprotective potentials. At 400 mg/kg the extract significantly reduced (p<0.05) the levels of AST (20.80±0.58), AST (36.80±0.73), and ALP (48.40±0.81) when compared with Silymarin. The study validates the traditional use of B. brieyi for treating liver disorders, showing that its extract and fractions have a dose-dependent effect in reducing the levels of ALT, AST, ALP, and other enzymes in carbon tetrachloride-induced liver damage in rats.
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Copyright (c) 2024 Ifeyinwa C. Ezeala, Onyinye Ezeala, Philip F. Uzor, Felix I. Nwafor, Anthony C. Ugwuja, Chimdinma E. Okoroafor, Emmanuel J. Uzodi, Ngozi J. Nwodo
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